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alexa fluor 594-conjugated ctb  (Thermo Fisher)


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    Structured Review

    Thermo Fisher alexa fluor 594-conjugated ctb
    Alexa Fluor 594 Conjugated Ctb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ctb-594/ctb+647/pm40536333-430-11-18
    Average 90 stars, based on 1 article reviews
    alexa fluor 594-conjugated ctb - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Injection:

    Article Title: CD47 protects synapses from excess microglia-mediated pruning during development
    Article Snippet: An unpaired t test was used to compare the degree of overlap in CD47KO and WT littermate controls at each threshold level Engulfment analysis Mice were injected with anterograde tracers (CTB-594 and CTB-647, ThermoFisher Scientific (C-22842, C-34778) in the left and right eyes, respectively, at P4 and their brains were harvested 24 hours later at P5 using the same methods as for immunohistochemistry.

    Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas
    Article Snippet: Murray Reardon et al., 2016 rAAV1/Sync-Flex-ChrimsonR-tdT UNC vector core Klapoetke et al., 2014 AAV1.CAG.flex.tdTomato Addgene Cat# 28306 AAV1.hSyn.GCaMP6f.WPRE.SV40 Addgene Cat# 100837 AAV1-syn-jGCaMP7b-WPRE Addgene Cat# 104489 ssAAV-1/2-hEF1a-mCherry-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V212-1 ssAAV-1/2-hEF1a-EGFP-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V211-1 ssAAV-1/2-hEF1a-EBFP2-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V213-1 Chemicals, peptides, and recombinant proteins DiI Thermo-Fisher Cat# D3911 DiO Thermo-Fisher Cat# V22886 CTB-488 Thermo-Fisher Cat# C34775 CTB-594 Thermo-Fisher Cat# C34777 Experimental models: Organisms/strains C57BL/6JRj Janvier Labs or Charles River N/A B6;129P2-Pvalbtm1(cre)Arbr/J The Jackson Laboratory JAX: 008069 Slc17a6tm2(cre)Lowl/J The Jackson Laboratory JAX: 016963 Software and algorithms ScanImage Vidrio Technologies, LLC Pologruto et al., 2003 Allen Common Coordinate Framework https://atlas.brain-map.org/ Wang et al., 2020 Kilosort https://github.com/MouseLand/Kilosort Pachitariu et al., 2016 Elastix https://elastix.lumc.nl/ Shamonin et al., 2014 Phy https://github.com/cortex-lab/phy BakingTray https://github.com/SainsburyWellcome Centre/BakingTray Campbell, 2020 Cellfinder https://github.com/brainglobe/cellfinder Tyson et al., 2020 scikit-image https://scikit-image.org/ Walt et al., 2014 matplotlib https://matplotlib.org/3.1.0/index.html Caswell et al., 2019 Fiji https://imagej.net/Fiji Schindelin et al., 2012 GPflow https://github.com/GPflow/GPflow Matthews et al., 2017 CircStat http://www.mathworks.com/matlabcentral/ fileexchange/10676-circular-statisticstoolbox-directional-statistics Berens, 2009 TimeSeriesAnalysis https://bitbucket.org/DylanMuir/ timeseriesanalysis/ Muir et al., 2020

    Article Title: Astrocytic synapse elimination controls ocular dominance plasticity
    Article Snippet: To label the right eye-derived retinogeniculate axon terminals, CTB-594 (1 mg/ml, Thermo Fisher) was intraorbitally injected into the right eye of the injected mice at P32.

    Article Title: An excitatory peri-tegmental reticular nucleus circuit for wake maintenance
    Article Snippet: 290 291 CTb tracing 292 Mice were injected unilaterally with 300 nL of CTb-594 (ThermoFisher, Cat #C34777) into the 293 LH and 300 nL of CTb-647 (ThermoFisher, Cat #34778) into the PZ using the above 294 coordinates.

    Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas
    Article Snippet: CTB-594 , Thermo-Fisher , Cat# C34777.

    Article Title: Medial geniculate body and primary auditory cortex differentially contribute to striatal sound representations
    Article Snippet: For retrograde labeling of MGB neurons projecting to ACx or auditory striatum, CTB-594 or CTB-488 (0.3 μl, ThermoFisher Scientific, Waltham, MA) was injected into the left ACx and auditory striatum, respectively.

    Expressing:

    Article Title: CD47 protects synapses from excess microglia-mediated pruning during development
    Article Snippet: An unpaired t test was used to compare the degree of overlap in CD47KO and WT littermate controls at each threshold level Engulfment analysis Mice were injected with anterograde tracers (CTB-594 and CTB-647, ThermoFisher Scientific (C-22842, C-34778) in the left and right eyes, respectively, at P4 and their brains were harvested 24 hours later at P5 using the same methods as for immunohistochemistry.

    Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas
    Article Snippet: Murray Reardon et al., 2016 rAAV1/Sync-Flex-ChrimsonR-tdT UNC vector core Klapoetke et al., 2014 AAV1.CAG.flex.tdTomato Addgene Cat# 28306 AAV1.hSyn.GCaMP6f.WPRE.SV40 Addgene Cat# 100837 AAV1-syn-jGCaMP7b-WPRE Addgene Cat# 104489 ssAAV-1/2-hEF1a-mCherry-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V212-1 ssAAV-1/2-hEF1a-EGFP-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V211-1 ssAAV-1/2-hEF1a-EBFP2-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V213-1 Chemicals, peptides, and recombinant proteins DiI Thermo-Fisher Cat# D3911 DiO Thermo-Fisher Cat# V22886 CTB-488 Thermo-Fisher Cat# C34775 CTB-594 Thermo-Fisher Cat# C34777 Experimental models: Organisms/strains C57BL/6JRj Janvier Labs or Charles River N/A B6;129P2-Pvalbtm1(cre)Arbr/J The Jackson Laboratory JAX: 008069 Slc17a6tm2(cre)Lowl/J The Jackson Laboratory JAX: 016963 Software and algorithms ScanImage Vidrio Technologies, LLC Pologruto et al., 2003 Allen Common Coordinate Framework https://atlas.brain-map.org/ Wang et al., 2020 Kilosort https://github.com/MouseLand/Kilosort Pachitariu et al., 2016 Elastix https://elastix.lumc.nl/ Shamonin et al., 2014 Phy https://github.com/cortex-lab/phy BakingTray https://github.com/SainsburyWellcome Centre/BakingTray Campbell, 2020 Cellfinder https://github.com/brainglobe/cellfinder Tyson et al., 2020 scikit-image https://scikit-image.org/ Walt et al., 2014 matplotlib https://matplotlib.org/3.1.0/index.html Caswell et al., 2019 Fiji https://imagej.net/Fiji Schindelin et al., 2012 GPflow https://github.com/GPflow/GPflow Matthews et al., 2017 CircStat http://www.mathworks.com/matlabcentral/ fileexchange/10676-circular-statisticstoolbox-directional-statistics Berens, 2009 TimeSeriesAnalysis https://bitbucket.org/DylanMuir/ timeseriesanalysis/ Muir et al., 2020

    Article Title: Astrocytic synapse elimination controls ocular dominance plasticity
    Article Snippet: To label the right eye-derived retinogeniculate axon terminals, CTB-594 (1 mg/ml, Thermo Fisher) was intraorbitally injected into the right eye of the injected mice at P32.

    Article Title: An excitatory peri-tegmental reticular nucleus circuit for wake maintenance
    Article Snippet: 290 291 CTb tracing 292 Mice were injected unilaterally with 300 nL of CTb-594 (ThermoFisher, Cat #C34777) into the 293 LH and 300 nL of CTb-647 (ThermoFisher, Cat #34778) into the PZ using the above 294 coordinates.

    Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas
    Article Snippet: CTB-594 , Thermo-Fisher , Cat# C34777.

    Article Title: Medial geniculate body and primary auditory cortex differentially contribute to striatal sound representations
    Article Snippet: For retrograde labeling of MGB neurons projecting to ACx or auditory striatum, CTB-594 or CTB-488 (0.3 μl, ThermoFisher Scientific, Waltham, MA) was injected into the left ACx and auditory striatum, respectively.

    Mouse Assay:

    Article Title: CD47 protects synapses from excess microglia-mediated pruning during development
    Article Snippet: An unpaired t test was used to compare the degree of overlap in CD47KO and WT littermate controls at each threshold level Engulfment analysis Mice were injected with anterograde tracers (CTB-594 and CTB-647, ThermoFisher Scientific (C-22842, C-34778) in the left and right eyes, respectively, at P4 and their brains were harvested 24 hours later at P5 using the same methods as for immunohistochemistry.

    Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas
    Article Snippet: Murray Reardon et al., 2016 rAAV1/Sync-Flex-ChrimsonR-tdT UNC vector core Klapoetke et al., 2014 AAV1.CAG.flex.tdTomato Addgene Cat# 28306 AAV1.hSyn.GCaMP6f.WPRE.SV40 Addgene Cat# 100837 AAV1-syn-jGCaMP7b-WPRE Addgene Cat# 104489 ssAAV-1/2-hEF1a-mCherry-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V212-1 ssAAV-1/2-hEF1a-EGFP-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V211-1 ssAAV-1/2-hEF1a-EBFP2-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V213-1 Chemicals, peptides, and recombinant proteins DiI Thermo-Fisher Cat# D3911 DiO Thermo-Fisher Cat# V22886 CTB-488 Thermo-Fisher Cat# C34775 CTB-594 Thermo-Fisher Cat# C34777 Experimental models: Organisms/strains C57BL/6JRj Janvier Labs or Charles River N/A B6;129P2-Pvalbtm1(cre)Arbr/J The Jackson Laboratory JAX: 008069 Slc17a6tm2(cre)Lowl/J The Jackson Laboratory JAX: 016963 Software and algorithms ScanImage Vidrio Technologies, LLC Pologruto et al., 2003 Allen Common Coordinate Framework https://atlas.brain-map.org/ Wang et al., 2020 Kilosort https://github.com/MouseLand/Kilosort Pachitariu et al., 2016 Elastix https://elastix.lumc.nl/ Shamonin et al., 2014 Phy https://github.com/cortex-lab/phy BakingTray https://github.com/SainsburyWellcome Centre/BakingTray Campbell, 2020 Cellfinder https://github.com/brainglobe/cellfinder Tyson et al., 2020 scikit-image https://scikit-image.org/ Walt et al., 2014 matplotlib https://matplotlib.org/3.1.0/index.html Caswell et al., 2019 Fiji https://imagej.net/Fiji Schindelin et al., 2012 GPflow https://github.com/GPflow/GPflow Matthews et al., 2017 CircStat http://www.mathworks.com/matlabcentral/ fileexchange/10676-circular-statisticstoolbox-directional-statistics Berens, 2009 TimeSeriesAnalysis https://bitbucket.org/DylanMuir/ timeseriesanalysis/ Muir et al., 2020

    Article Title: Astrocytic synapse elimination controls ocular dominance plasticity
    Article Snippet: To label the right eye-derived retinogeniculate axon terminals, CTB-594 (1 mg/ml, Thermo Fisher) was intraorbitally injected into the right eye of the injected mice at P32.

    Article Title: An excitatory peri-tegmental reticular nucleus circuit for wake maintenance
    Article Snippet: 290 291 CTb tracing 292 Mice were injected unilaterally with 300 nL of CTb-594 (ThermoFisher, Cat #C34777) into the 293 LH and 300 nL of CTb-647 (ThermoFisher, Cat #34778) into the PZ using the above 294 coordinates.

    Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas
    Article Snippet: CTB-594 , Thermo-Fisher , Cat# C34777.

    Article Title: Medial geniculate body and primary auditory cortex differentially contribute to striatal sound representations
    Article Snippet: For retrograde labeling of MGB neurons projecting to ACx or auditory striatum, CTB-594 or CTB-488 (0.3 μl, ThermoFisher Scientific, Waltham, MA) was injected into the left ACx and auditory striatum, respectively.

    Plasmid Preparation:

    Article Title: CD47 protects synapses from excess microglia-mediated pruning during development
    Article Snippet: An unpaired t test was used to compare the degree of overlap in CD47KO and WT littermate controls at each threshold level Engulfment analysis Mice were injected with anterograde tracers (CTB-594 and CTB-647, ThermoFisher Scientific (C-22842, C-34778) in the left and right eyes, respectively, at P4 and their brains were harvested 24 hours later at P5 using the same methods as for immunohistochemistry.

    Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas
    Article Snippet: Murray Reardon et al., 2016 rAAV1/Sync-Flex-ChrimsonR-tdT UNC vector core Klapoetke et al., 2014 AAV1.CAG.flex.tdTomato Addgene Cat# 28306 AAV1.hSyn.GCaMP6f.WPRE.SV40 Addgene Cat# 100837 AAV1-syn-jGCaMP7b-WPRE Addgene Cat# 104489 ssAAV-1/2-hEF1a-mCherry-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V212-1 ssAAV-1/2-hEF1a-EGFP-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V211-1 ssAAV-1/2-hEF1a-EBFP2-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V213-1 Chemicals, peptides, and recombinant proteins DiI Thermo-Fisher Cat# D3911 DiO Thermo-Fisher Cat# V22886 CTB-488 Thermo-Fisher Cat# C34775 CTB-594 Thermo-Fisher Cat# C34777 Experimental models: Organisms/strains C57BL/6JRj Janvier Labs or Charles River N/A B6;129P2-Pvalbtm1(cre)Arbr/J The Jackson Laboratory JAX: 008069 Slc17a6tm2(cre)Lowl/J The Jackson Laboratory JAX: 016963 Software and algorithms ScanImage Vidrio Technologies, LLC Pologruto et al., 2003 Allen Common Coordinate Framework https://atlas.brain-map.org/ Wang et al., 2020 Kilosort https://github.com/MouseLand/Kilosort Pachitariu et al., 2016 Elastix https://elastix.lumc.nl/ Shamonin et al., 2014 Phy https://github.com/cortex-lab/phy BakingTray https://github.com/SainsburyWellcome Centre/BakingTray Campbell, 2020 Cellfinder https://github.com/brainglobe/cellfinder Tyson et al., 2020 scikit-image https://scikit-image.org/ Walt et al., 2014 matplotlib https://matplotlib.org/3.1.0/index.html Caswell et al., 2019 Fiji https://imagej.net/Fiji Schindelin et al., 2012 GPflow https://github.com/GPflow/GPflow Matthews et al., 2017 CircStat http://www.mathworks.com/matlabcentral/ fileexchange/10676-circular-statisticstoolbox-directional-statistics Berens, 2009 TimeSeriesAnalysis https://bitbucket.org/DylanMuir/ timeseriesanalysis/ Muir et al., 2020

    Article Title: Astrocytic synapse elimination controls ocular dominance plasticity
    Article Snippet: To label the right eye-derived retinogeniculate axon terminals, CTB-594 (1 mg/ml, Thermo Fisher) was intraorbitally injected into the right eye of the injected mice at P32.

    Article Title: An excitatory peri-tegmental reticular nucleus circuit for wake maintenance
    Article Snippet: 290 291 CTb tracing 292 Mice were injected unilaterally with 300 nL of CTb-594 (ThermoFisher, Cat #C34777) into the 293 LH and 300 nL of CTb-647 (ThermoFisher, Cat #34778) into the PZ using the above 294 coordinates.

    Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas
    Article Snippet: CTB-594 , Thermo-Fisher , Cat# C34777.

    Article Title: Medial geniculate body and primary auditory cortex differentially contribute to striatal sound representations
    Article Snippet: For retrograde labeling of MGB neurons projecting to ACx or auditory striatum, CTB-594 or CTB-488 (0.3 μl, ThermoFisher Scientific, Waltham, MA) was injected into the left ACx and auditory striatum, respectively.

    Recombinant:

    Article Title: CD47 protects synapses from excess microglia-mediated pruning during development
    Article Snippet: An unpaired t test was used to compare the degree of overlap in CD47KO and WT littermate controls at each threshold level Engulfment analysis Mice were injected with anterograde tracers (CTB-594 and CTB-647, ThermoFisher Scientific (C-22842, C-34778) in the left and right eyes, respectively, at P4 and their brains were harvested 24 hours later at P5 using the same methods as for immunohistochemistry.

    Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas
    Article Snippet: Murray Reardon et al., 2016 rAAV1/Sync-Flex-ChrimsonR-tdT UNC vector core Klapoetke et al., 2014 AAV1.CAG.flex.tdTomato Addgene Cat# 28306 AAV1.hSyn.GCaMP6f.WPRE.SV40 Addgene Cat# 100837 AAV1-syn-jGCaMP7b-WPRE Addgene Cat# 104489 ssAAV-1/2-hEF1a-mCherry-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V212-1 ssAAV-1/2-hEF1a-EGFP-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V211-1 ssAAV-1/2-hEF1a-EBFP2-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V213-1 Chemicals, peptides, and recombinant proteins DiI Thermo-Fisher Cat# D3911 DiO Thermo-Fisher Cat# V22886 CTB-488 Thermo-Fisher Cat# C34775 CTB-594 Thermo-Fisher Cat# C34777 Experimental models: Organisms/strains C57BL/6JRj Janvier Labs or Charles River N/A B6;129P2-Pvalbtm1(cre)Arbr/J The Jackson Laboratory JAX: 008069 Slc17a6tm2(cre)Lowl/J The Jackson Laboratory JAX: 016963 Software and algorithms ScanImage Vidrio Technologies, LLC Pologruto et al., 2003 Allen Common Coordinate Framework https://atlas.brain-map.org/ Wang et al., 2020 Kilosort https://github.com/MouseLand/Kilosort Pachitariu et al., 2016 Elastix https://elastix.lumc.nl/ Shamonin et al., 2014 Phy https://github.com/cortex-lab/phy BakingTray https://github.com/SainsburyWellcome Centre/BakingTray Campbell, 2020 Cellfinder https://github.com/brainglobe/cellfinder Tyson et al., 2020 scikit-image https://scikit-image.org/ Walt et al., 2014 matplotlib https://matplotlib.org/3.1.0/index.html Caswell et al., 2019 Fiji https://imagej.net/Fiji Schindelin et al., 2012 GPflow https://github.com/GPflow/GPflow Matthews et al., 2017 CircStat http://www.mathworks.com/matlabcentral/ fileexchange/10676-circular-statisticstoolbox-directional-statistics Berens, 2009 TimeSeriesAnalysis https://bitbucket.org/DylanMuir/ timeseriesanalysis/ Muir et al., 2020

    Article Title: Astrocytic synapse elimination controls ocular dominance plasticity
    Article Snippet: To label the right eye-derived retinogeniculate axon terminals, CTB-594 (1 mg/ml, Thermo Fisher) was intraorbitally injected into the right eye of the injected mice at P32.

    Article Title: An excitatory peri-tegmental reticular nucleus circuit for wake maintenance
    Article Snippet: 290 291 CTb tracing 292 Mice were injected unilaterally with 300 nL of CTb-594 (ThermoFisher, Cat #C34777) into the 293 LH and 300 nL of CTb-647 (ThermoFisher, Cat #34778) into the PZ using the above 294 coordinates.

    Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas
    Article Snippet: CTB-594 , Thermo-Fisher , Cat# C34777.

    Article Title: Medial geniculate body and primary auditory cortex differentially contribute to striatal sound representations
    Article Snippet: For retrograde labeling of MGB neurons projecting to ACx or auditory striatum, CTB-594 or CTB-488 (0.3 μl, ThermoFisher Scientific, Waltham, MA) was injected into the left ACx and auditory striatum, respectively.

    Software:

    Article Title: CD47 protects synapses from excess microglia-mediated pruning during development
    Article Snippet: An unpaired t test was used to compare the degree of overlap in CD47KO and WT littermate controls at each threshold level Engulfment analysis Mice were injected with anterograde tracers (CTB-594 and CTB-647, ThermoFisher Scientific (C-22842, C-34778) in the left and right eyes, respectively, at P4 and their brains were harvested 24 hours later at P5 using the same methods as for immunohistochemistry.

    Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas
    Article Snippet: Murray Reardon et al., 2016 rAAV1/Sync-Flex-ChrimsonR-tdT UNC vector core Klapoetke et al., 2014 AAV1.CAG.flex.tdTomato Addgene Cat# 28306 AAV1.hSyn.GCaMP6f.WPRE.SV40 Addgene Cat# 100837 AAV1-syn-jGCaMP7b-WPRE Addgene Cat# 104489 ssAAV-1/2-hEF1a-mCherry-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V212-1 ssAAV-1/2-hEF1a-EGFP-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V211-1 ssAAV-1/2-hEF1a-EBFP2-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V213-1 Chemicals, peptides, and recombinant proteins DiI Thermo-Fisher Cat# D3911 DiO Thermo-Fisher Cat# V22886 CTB-488 Thermo-Fisher Cat# C34775 CTB-594 Thermo-Fisher Cat# C34777 Experimental models: Organisms/strains C57BL/6JRj Janvier Labs or Charles River N/A B6;129P2-Pvalbtm1(cre)Arbr/J The Jackson Laboratory JAX: 008069 Slc17a6tm2(cre)Lowl/J The Jackson Laboratory JAX: 016963 Software and algorithms ScanImage Vidrio Technologies, LLC Pologruto et al., 2003 Allen Common Coordinate Framework https://atlas.brain-map.org/ Wang et al., 2020 Kilosort https://github.com/MouseLand/Kilosort Pachitariu et al., 2016 Elastix https://elastix.lumc.nl/ Shamonin et al., 2014 Phy https://github.com/cortex-lab/phy BakingTray https://github.com/SainsburyWellcome Centre/BakingTray Campbell, 2020 Cellfinder https://github.com/brainglobe/cellfinder Tyson et al., 2020 scikit-image https://scikit-image.org/ Walt et al., 2014 matplotlib https://matplotlib.org/3.1.0/index.html Caswell et al., 2019 Fiji https://imagej.net/Fiji Schindelin et al., 2012 GPflow https://github.com/GPflow/GPflow Matthews et al., 2017 CircStat http://www.mathworks.com/matlabcentral/ fileexchange/10676-circular-statisticstoolbox-directional-statistics Berens, 2009 TimeSeriesAnalysis https://bitbucket.org/DylanMuir/ timeseriesanalysis/ Muir et al., 2020

    Article Title: Astrocytic synapse elimination controls ocular dominance plasticity
    Article Snippet: To label the right eye-derived retinogeniculate axon terminals, CTB-594 (1 mg/ml, Thermo Fisher) was intraorbitally injected into the right eye of the injected mice at P32.

    Article Title: An excitatory peri-tegmental reticular nucleus circuit for wake maintenance
    Article Snippet: 290 291 CTb tracing 292 Mice were injected unilaterally with 300 nL of CTb-594 (ThermoFisher, Cat #C34777) into the 293 LH and 300 nL of CTb-647 (ThermoFisher, Cat #34778) into the PZ using the above 294 coordinates.

    Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas
    Article Snippet: CTB-594 , Thermo-Fisher , Cat# C34777.

    Article Title: Medial geniculate body and primary auditory cortex differentially contribute to striatal sound representations
    Article Snippet: For retrograde labeling of MGB neurons projecting to ACx or auditory striatum, CTB-594 or CTB-488 (0.3 μl, ThermoFisher Scientific, Waltham, MA) was injected into the left ACx and auditory striatum, respectively.

    Immunohistochemistry:

    Article Title: CD47 protects synapses from excess microglia-mediated pruning during development
    Article Snippet: An unpaired t test was used to compare the degree of overlap in CD47KO and WT littermate controls at each threshold level Engulfment analysis Mice were injected with anterograde tracers (CTB-594 and CTB-647, ThermoFisher Scientific (C-22842, C-34778) in the left and right eyes, respectively, at P4 and their brains were harvested 24 hours later at P5 using the same methods as for immunohistochemistry.

    Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas
    Article Snippet: Murray Reardon et al., 2016 rAAV1/Sync-Flex-ChrimsonR-tdT UNC vector core Klapoetke et al., 2014 AAV1.CAG.flex.tdTomato Addgene Cat# 28306 AAV1.hSyn.GCaMP6f.WPRE.SV40 Addgene Cat# 100837 AAV1-syn-jGCaMP7b-WPRE Addgene Cat# 104489 ssAAV-1/2-hEF1a-mCherry-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V212-1 ssAAV-1/2-hEF1a-EGFP-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V211-1 ssAAV-1/2-hEF1a-EBFP2-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V213-1 Chemicals, peptides, and recombinant proteins DiI Thermo-Fisher Cat# D3911 DiO Thermo-Fisher Cat# V22886 CTB-488 Thermo-Fisher Cat# C34775 CTB-594 Thermo-Fisher Cat# C34777 Experimental models: Organisms/strains C57BL/6JRj Janvier Labs or Charles River N/A B6;129P2-Pvalbtm1(cre)Arbr/J The Jackson Laboratory JAX: 008069 Slc17a6tm2(cre)Lowl/J The Jackson Laboratory JAX: 016963 Software and algorithms ScanImage Vidrio Technologies, LLC Pologruto et al., 2003 Allen Common Coordinate Framework https://atlas.brain-map.org/ Wang et al., 2020 Kilosort https://github.com/MouseLand/Kilosort Pachitariu et al., 2016 Elastix https://elastix.lumc.nl/ Shamonin et al., 2014 Phy https://github.com/cortex-lab/phy BakingTray https://github.com/SainsburyWellcome Centre/BakingTray Campbell, 2020 Cellfinder https://github.com/brainglobe/cellfinder Tyson et al., 2020 scikit-image https://scikit-image.org/ Walt et al., 2014 matplotlib https://matplotlib.org/3.1.0/index.html Caswell et al., 2019 Fiji https://imagej.net/Fiji Schindelin et al., 2012 GPflow https://github.com/GPflow/GPflow Matthews et al., 2017 CircStat http://www.mathworks.com/matlabcentral/ fileexchange/10676-circular-statisticstoolbox-directional-statistics Berens, 2009 TimeSeriesAnalysis https://bitbucket.org/DylanMuir/ timeseriesanalysis/ Muir et al., 2020

    Article Title: Astrocytic synapse elimination controls ocular dominance plasticity
    Article Snippet: To label the right eye-derived retinogeniculate axon terminals, CTB-594 (1 mg/ml, Thermo Fisher) was intraorbitally injected into the right eye of the injected mice at P32.

    Article Title: An excitatory peri-tegmental reticular nucleus circuit for wake maintenance
    Article Snippet: 290 291 CTb tracing 292 Mice were injected unilaterally with 300 nL of CTb-594 (ThermoFisher, Cat #C34777) into the 293 LH and 300 nL of CTb-647 (ThermoFisher, Cat #34778) into the PZ using the above 294 coordinates.

    Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas
    Article Snippet: CTB-594 , Thermo-Fisher , Cat# C34777.

    Article Title: Medial geniculate body and primary auditory cortex differentially contribute to striatal sound representations
    Article Snippet: For retrograde labeling of MGB neurons projecting to ACx or auditory striatum, CTB-594 or CTB-488 (0.3 μl, ThermoFisher Scientific, Waltham, MA) was injected into the left ACx and auditory striatum, respectively.

    Labeling:

    Article Title: CD47 protects synapses from excess microglia-mediated pruning during development
    Article Snippet: An unpaired t test was used to compare the degree of overlap in CD47KO and WT littermate controls at each threshold level Engulfment analysis Mice were injected with anterograde tracers (CTB-594 and CTB-647, ThermoFisher Scientific (C-22842, C-34778) in the left and right eyes, respectively, at P4 and their brains were harvested 24 hours later at P5 using the same methods as for immunohistochemistry.

    Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas
    Article Snippet: Murray Reardon et al., 2016 rAAV1/Sync-Flex-ChrimsonR-tdT UNC vector core Klapoetke et al., 2014 AAV1.CAG.flex.tdTomato Addgene Cat# 28306 AAV1.hSyn.GCaMP6f.WPRE.SV40 Addgene Cat# 100837 AAV1-syn-jGCaMP7b-WPRE Addgene Cat# 104489 ssAAV-1/2-hEF1a-mCherry-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V212-1 ssAAV-1/2-hEF1a-EGFP-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V211-1 ssAAV-1/2-hEF1a-EBFP2-WPRE-bGHp(A) ETH Zurich VVF – plasmid from addgene V213-1 Chemicals, peptides, and recombinant proteins DiI Thermo-Fisher Cat# D3911 DiO Thermo-Fisher Cat# V22886 CTB-488 Thermo-Fisher Cat# C34775 CTB-594 Thermo-Fisher Cat# C34777 Experimental models: Organisms/strains C57BL/6JRj Janvier Labs or Charles River N/A B6;129P2-Pvalbtm1(cre)Arbr/J The Jackson Laboratory JAX: 008069 Slc17a6tm2(cre)Lowl/J The Jackson Laboratory JAX: 016963 Software and algorithms ScanImage Vidrio Technologies, LLC Pologruto et al., 2003 Allen Common Coordinate Framework https://atlas.brain-map.org/ Wang et al., 2020 Kilosort https://github.com/MouseLand/Kilosort Pachitariu et al., 2016 Elastix https://elastix.lumc.nl/ Shamonin et al., 2014 Phy https://github.com/cortex-lab/phy BakingTray https://github.com/SainsburyWellcome Centre/BakingTray Campbell, 2020 Cellfinder https://github.com/brainglobe/cellfinder Tyson et al., 2020 scikit-image https://scikit-image.org/ Walt et al., 2014 matplotlib https://matplotlib.org/3.1.0/index.html Caswell et al., 2019 Fiji https://imagej.net/Fiji Schindelin et al., 2012 GPflow https://github.com/GPflow/GPflow Matthews et al., 2017 CircStat http://www.mathworks.com/matlabcentral/ fileexchange/10676-circular-statisticstoolbox-directional-statistics Berens, 2009 TimeSeriesAnalysis https://bitbucket.org/DylanMuir/ timeseriesanalysis/ Muir et al., 2020

    Article Title: Astrocytic synapse elimination controls ocular dominance plasticity
    Article Snippet: To label the right eye-derived retinogeniculate axon terminals, CTB-594 (1 mg/ml, Thermo Fisher) was intraorbitally injected into the right eye of the injected mice at P32.

    Article Title: An excitatory peri-tegmental reticular nucleus circuit for wake maintenance
    Article Snippet: 290 291 CTb tracing 292 Mice were injected unilaterally with 300 nL of CTb-594 (ThermoFisher, Cat #C34777) into the 293 LH and 300 nL of CTb-647 (ThermoFisher, Cat #34778) into the PZ using the above 294 coordinates.

    Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas
    Article Snippet: CTB-594 , Thermo-Fisher , Cat# C34777.

    Article Title: Medial geniculate body and primary auditory cortex differentially contribute to striatal sound representations
    Article Snippet: For retrograde labeling of MGB neurons projecting to ACx or auditory striatum, CTB-594 or CTB-488 (0.3 μl, ThermoFisher Scientific, Waltham, MA) was injected into the left ACx and auditory striatum, respectively.



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    Experiment 1: CTb labelling of IL-NAc projections after initial oxycodone learning. (A) Depiction of the placement of CTb-488 and <t>CTb-594</t> injections into the NAc core and shell subregions. (B) Representative images of Fos, CTb-594, CTb-488 and Fos + CTb-594 + CTb-488 fluorescence in the IL of rats killed from the home cage (no test) compared with rats killed following the oxycodone-seeking test (test). The scale bars represent 100 μm. (C) Average number of Fos+ cells/mm2 (Fos expression) in the IL of rats killed from the home cage (no test) compared with rats killed after the oxycodone-seeking test (test). (D) Percentage of Fos+ cells colabelled with CTb-488 in the IL of rats killed from the home cage (no test) compared with rats killed after the oxycodone-seeking test (test). (E) Percentage of Fos+ cells colabelled with CTb-594 in the IL of rats killed from the home cage (no test) compared with rats killed after the oxycodone-seeking test (test). Data are presented as mean ± SEM (n = 9–10 per group). *p < 0.05 compared with no test controls
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    Image Search Results


    Experiment 1: CTb labelling of IL-NAc projections after initial oxycodone learning. (A) Depiction of the placement of CTb-488 and CTb-594 injections into the NAc core and shell subregions. (B) Representative images of Fos, CTb-594, CTb-488 and Fos + CTb-594 + CTb-488 fluorescence in the IL of rats killed from the home cage (no test) compared with rats killed following the oxycodone-seeking test (test). The scale bars represent 100 μm. (C) Average number of Fos+ cells/mm2 (Fos expression) in the IL of rats killed from the home cage (no test) compared with rats killed after the oxycodone-seeking test (test). (D) Percentage of Fos+ cells colabelled with CTb-488 in the IL of rats killed from the home cage (no test) compared with rats killed after the oxycodone-seeking test (test). (E) Percentage of Fos+ cells colabelled with CTb-594 in the IL of rats killed from the home cage (no test) compared with rats killed after the oxycodone-seeking test (test). Data are presented as mean ± SEM (n = 9–10 per group). *p < 0.05 compared with no test controls

    Journal: Addiction biology

    Article Title: Fos-expressing neuronal ensembles in rat infralimbic cortex encode initial and maintained oxycodone seeking in rats

    doi: 10.1111/adb.13148

    Figure Lengend Snippet: Experiment 1: CTb labelling of IL-NAc projections after initial oxycodone learning. (A) Depiction of the placement of CTb-488 and CTb-594 injections into the NAc core and shell subregions. (B) Representative images of Fos, CTb-594, CTb-488 and Fos + CTb-594 + CTb-488 fluorescence in the IL of rats killed from the home cage (no test) compared with rats killed following the oxycodone-seeking test (test). The scale bars represent 100 μm. (C) Average number of Fos+ cells/mm2 (Fos expression) in the IL of rats killed from the home cage (no test) compared with rats killed after the oxycodone-seeking test (test). (D) Percentage of Fos+ cells colabelled with CTb-488 in the IL of rats killed from the home cage (no test) compared with rats killed after the oxycodone-seeking test (test). (E) Percentage of Fos+ cells colabelled with CTb-594 in the IL of rats killed from the home cage (no test) compared with rats killed after the oxycodone-seeking test (test). Data are presented as mean ± SEM (n = 9–10 per group). *p < 0.05 compared with no test controls

    Article Snippet: Intracranial CTb injections In Experiment 1, we contralaterally microinfused (0.3 μl per side) fluorescent CTb-488 (50 μg/μl; Thermo Fisher, Ref: C22841, Lot: 2155272) into the NAc core [anteroposterior: +1.6, mediolateral: ±2.5, and dorsoventral: −6.5 (10° angle)] and CTb-594 (50 μg/μl; Thermo Fisher, Ref: C22842, Lot: 2160063) into the NAc shell [anteroposterior: +1.6, mediolateral: ±2.5 and dorsoventral: −7.3 (10° angle)] using a Micro4 Microsyringe Pump Controller (World Precision Instruments) and 10-μl Hamilton syringes.

    Techniques: Fluorescence, Expressing